Tandem immunoaffinity purification of protein complexes from Caenorhabditis elegans.
نویسندگان
چکیده
منابع مشابه
Immunoaffinity purification of avermectin-binding proteins from the free-living nematode Caenorhabditis elegans and the fruitfly Drosophila melanogaster.
Avermectin-binding proteins from the free-living nematode worm Caenorhabditis elegans and from the fruitfly Drosophila melanogaster were purified to homogeneity via a three-step procedure. The binding proteins were covalently labelled using a radioactive photoaffinity probe and then partially purified on a Sephacryl S-300 gel-filtration column. The radiolabelled binding proteins were then purif...
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ورودعنوان ژورنال:
- BioTechniques
دوره 36 5 شماره
صفحات -
تاریخ انتشار 2004